The pore is completely plugged towards its periplasmic end, though, by the presence of a large 150-residue N-terminal domain, which consists mainly of a four-stranded β-sheet. Two loops from this ...

In a recent study published in Engineering, researchers have made significant strides in the enzymatic depolymerization of polyethylene terephthalate (PET) through β-sheet engineering of IsPETase.

As reported in their study, they developed a promising approach to produce four-stranded β-sheets using silver atoms as metal–peptide coordination centers. The researchers engineered a ...